Leptin anticorps

N° du produit ABIN181608

L’anticorps Chèvre Polyclonal anti-Leptin a été validé pour WB et EIA. Il convient pour détecter Leptin dans des échantillons de Rat.

Aperçu rapide pour Leptin anticorps (ABIN181608)

Antigène: Leptin (LEP)

Reactivité: Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Leptin est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Leptin anticorps

Specificité: This antibody detects Leptin.

Purification: Immunoaffinity chromatography

Immunogène: Highly pure (> 98 %) recombinant Rat Leptin

Information d'application

Indications d'application: ELISA: Indirect: To detect Rat Leptin by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant Rat Leptin. Sandwich: To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-RatLeptin as a detection antibody, this anibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin. Western Blot: To detect Rat Leptin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur Leptin

Antigène: Leptin (LEP)

Autre désignation: Leptin

Sujet: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

ID gène: 25608

NCBI Accession: NP_037208

UniProt: P50596

Pathways: Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process